Cre/loxP system
true
<a href='https://brc.riken.jp/mus/pcr02884'>Genotyping protocol -PCR-</a>
C (3-6 months)
FVB/N-Tg(Pitx3-cre/ESR1)1Ydq transgenic mice was made by inserting CreER into the Pitx3 ATG locus of a mouse BAC clone.
RBRC02884
FVB/N-Tg(Pitx3-cre/ESR1)1Ydq transgenic mice was made by inserting CreER into the Pitx3 ATG locus of a mouse BAC clone.
mouse Pitx3 promoter, Phage P1 cre recombinase gene, human estrogen receptor 1 cDNA, bovine growth hormone polyA, yeast FRT (flipase recombination target) site
FLP/frt system
The transgene was microinjected into FVB fertilized eggs, then later was crossed to C57BL/6 for two generations.
The transgene was microinjected into FVB fertilized eggs, then later was crossed to C57BL/6 for two generations.
Tg(Pitx3-cre/ESR1)1Ydq; FVB/N-Tg(Pitx3-cre/ESR1)1Yqd
Tg(Pitx3-cre/ESR1)1Ydq; FVB/N-Tg(Pitx3-cre/ESR1)1Yqd
Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 46, 324-328 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Yu-Qiang Ding
Yu-Qiang Ding
C(3〜6か月)
Viable and fertile with no obvious abnormalities.
Viable and fertile with no obvious abnormalities.
条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Genesis, 46, 324-328 (2008).<br>研究成果の公表にあたって謝辞の表明を必要とする。
FVB/N-Tg(Pitx3-cre/ESR1)1Yqd
FVB/N-Tg(Pitx3-cre/ESR1)1Yqd